汎用品年度末キャンペーン実施中 ~ 2018年3月31日まで
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情報全件数:40件
21件~30件を表示
製品名
 
製品簡略説明
商品コード:80-001      在庫切れ

本キットは、サンドイッチELISA(Enzyme Linked Immunosorbent Assay)法により、細胞培養液等のHIV-1 Gag p24抗原量を簡便に測定できるキットです。p24抗原はHIV-1ウイルスの構造タンパク質で、このタンパク質を測定することにより試料中のウイルス量を推定することができます。このキットはHIV-1由来のLentivirus ベクターの定量にも使えます。

[測定原理]
抗体固相化プレートを固相とし、ビオチン化抗体と酵素標識ストレプトアビジンを用いるサンドイッチ法です。
 
 
商品コード
容量
価格
 
80-001
96 assay
60,000円(税抜)

製品名
 
製品簡略説明
製品番号:65-155

Background: Matrix protein 1 (M1) plays critical roles in virus replication from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1 a non-raft-associated protein into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion where it binds the RNP. During virus entry into cell the M2 ion channel acidifies the internal virion core inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus where viral transcription and replication can take place.
M1 of Influenza B virus consists of 248 amino acids with molecular mass of 27415.
Applications
1) Western blotting (1/500~1/1000 dilution)
2) Immunofluorescent and Immunocytochemical staining (1/100~1/200 dilution)
3) Immunoprecipitation (1/200 dilution)
4) ELISA (assay dependent)

 
 
商品コード
容量
価格
 
65-155
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
製品番号:65-160

Background: Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2 releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Post-translational modificationi
HAo is consists of 584 amino acids with molecular mass of 63275. In natural infection inactive HA is matured into HA1 and HA2. By sequence similarity it is indicated to be palmitoylated.
 
 
商品コード
容量
価格
 
65-160
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
製品番号:65-165

Background: Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2 releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Post-translational modificationi
HAo is consists of 584 amino acids with molecular mass of 63275. In natural infection inactive HA is matured into HA1 and HA2. By the sequence similarity it is indicated to be palmitoylated.
 
 
商品コード
容量
価格
 
65-165
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
製品番号:65-170

Background: Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly expressed during the course of infection. It is a structural protein which encapsidates the negative strand viral RNA and is essential for RNA transcription replication and packaging. From the nucleotide sequence NP
is consists of 560 amino acids with calculated molecular mass of 61770.

Post-translational modification: Late in virus-infected cells may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.
 
 
商品コード
容量
価格
 
65-170
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
商品コード:65-350

Varicella Zoster Virus (VZV) is one of eight herpesviruses known to infect humans and vertebrates. VZV only affects humans and commonly causes chickenpox in children teens and young adults and herpes zoster (shingles) in adults and rarely in children. VZV is known by many names including chickenpox virus varicella virus zoster virus and human herpesvirus type 3 (HHV-3).
VZV infects the nerves and causes a wide variety of symptoms. After the primary infection (chickenpox) the virus goes dormant in the nerves including the cranial nerve ganglia dorsal root ganglia and autonomic ganglia. Many years after the patient has recovered from chickenpox VZV can reactivate to cause a number of neurologic conditions
Immediate early protein 62 (IE62) of is major transcriptional transactivator of VZV. It may interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. It interacts with IE4 and IE63 and with host USF and Sp1.It negatively regulates its own transcription. This immediate early protein may be necessary in virion for viral pathogenesis.
IE62 consists of 1310 amino acids with 140 kDa mass. It is phosphorylated by ORF66 protein kinase on Ser-686 and Ser-722. It is also phosphorylated by ORF47 protein kinase and human CSNK2A1/CKII.SK

 
 
商品コード
容量
価格
 
65-350
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
商品コード:65-353

Varicella Zoster Virus (VZV) is one of eight herpesviruses known to infect humans and vertebrates. VZV only affects humans and commonly causes chickenpox in children teens and young adults and herpes zoster (shingles) in adults and rarely in children. VZV is known by many names including chickenpox virus varicella virus zoster virus and human herpesvirus type 3 (HHV-3).
VZV infects the nerves and causes a wide variety of symptoms. After the primary infection (chickenpox) the virus goes dormant in the nerves including the cranial nerve ganglia dorsal root ganglia and autonomic ganglia. Many years after the patient has recovered from chickenpox VZV can reactivate to cause a number of neurologic conditions
Immediate early protein 62 (IE62) of is major transcriptional transactivator of VZV. It may interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. It interacts with IE4 and IE63 and with host USF and Sp1.It negatively regulates its own transcription. This immediate early protein may be necessary in virion for viral pathogenesis.
IE62 consists of 1310 amino acids with 140 kDa mass. It is phosphorylated by ORF66 protein kinase on Ser-686 and Ser-722. It is also phosphorylated by ORF47 protein kinase and human CSNK2A1/CKII.SK
 
 
商品コード
容量
価格
 
65-353
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
商品コード:65-358

Shipping and Storage temperature: Ship at 4℃ and store at -20℃. Do not
freeze.
Immunogen: Varicella-zoster virus Oka vaccine strain
Specificity: Reacts with gE of VZV

Applications
1) Western blotting (1/2000-1/5000)
2) Immunoprecipitation (1/100)
3) Imunofluorescence staining and Immunocytochemistry (1/50-1/100)
4) ELISA (1/5000)

Isotype: mouse IgG2a kappa
Product:Purified by chromatography
Form: 1 mg/ml in PBS 50% glycerol filter sterilized. Azide- and carrier-free.
 
 
商品コード
容量
価格
 
65-358
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
商品コード:65-363

Varicella Zoster Virus (VZV) is one of eight herpesviruses known to infect humans and vertebrates. VZV only affects humans and commonly causes chickenpox in children teens and young adults and herpes zoster (shingles) in adults and rarely in children. VZV is known by many names including chickenpox virus varicella virus zoster virus and human herpesvirus type 3 (HHV-3).
VZV infects the nerves and causes a wide variety of symptoms. After the primary infection (chickenpox) the virus goes dormant in the nerves including the cranial nerve ganglia dorsal root ganglia and autonomic ganglia. Many years after the patient has recovered from chickenpox VZV can reactivate to cause a number of neurologic conditions
The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding of gD to one of its receptors membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis
gH is consists of 841 amino acids with molecular mass of 94 kDa
 
 
商品コード
容量
価格
 
65-363
100 ug
45,000円(税抜)

製品名
 
製品簡略説明
商品コード:65-001
性状:0.09%アジ化ナトリウム添加抗血清
保存:-20℃
関連製品:HIV-1逆転写酵素(05-001、05-002)
 
 
商品コード
容量
価格
 
65-001
100 ul
39,000円(税抜)

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