抗Rpn12(S. cerevisiae)抗体、ウサギPC精製抗体

商品コード:62-209

プロテアソームはユビキチンで修飾されたタンパク質を分解する酵素である。ビキチン-プロテアソームによる分解システムは、傷害を受けたりミスフォールドしたタンパク質を除去するのみならず、細胞周期制御、免疫応答、シグナル伝達といった細胞内の様々な働きに関わっている(文献1)。活性型の26Sプロテアソームはタンパク質分解実行ユニットである20Sプロテアソームの両端に、それを制御する19S複合体が会合した巨大な分子複合体である。19S複合体はさらにbase（基部）とlid（蓋部）に分けられる。Rpn12 はlidのnon-ATPase サブユニットのひとつで、基部のATPase サブユニットであるRpt1と相互作用する。Rpn12とRpt1の二重変異株では致死的となり、Rpn12とRpt1 の強い相互関係が示唆される。Rpn12 とRpt1の二重変異株では26Sプロテアソームの機能が完全に阻害される。

抗Leaf-FNR1抗体、ウサギポリクロ

商品コード:81-001

Background: Ferredoxin-NADP reductase, leaf isozyme 1 (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.

抗Swi6(S. pombe) 抗体、ウサギPC抗血清

商品コード:63-101

分裂酵母のSwi651タンパク質は動物のHP1 (Hetrochromatin Protein 1)の機能的、構造的ホモログで、セントロメア、テロメア、サイレントな接合型遺伝子座に結合して、ヘテロクロマチンの形成に関与する。Swi6はLys9がメチル化されたヒストンH３やコヒーシンのサブユニットPsc3共結合して遺伝子サイレンシングや姉妹染色体の接合にも関与する (文献1)。

　本品は大腸菌で組換え体タンパク質として発現させて、高度に精製した全長のS. pombe Swi6タンパク質(His-Tag付)を抗原としてウサギを免疫して作成した。この抗血清を用いて、分裂酵母粗抽出液を用いたウェスタンブロッテイング法でSwi6は分子量が37 kDにも関わらず～53 kDの位置に検出される（図1）（文献23）。

抗-Nup98抗体、マウスモノクローナル (13C2)

製品番号：70-345

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition Nup98 plays roles in several important biological events such as gene expression mitotic checkpoint and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation.
In ciliates a unicellular organism having two functionally distinct nuclei GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus and these different Nup98s participate in a nucleus-selective transport mechanism.

抗-Nup98抗体、マウスモノクローナル (21A10)

製品番号：70-346

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition Nup98 plays roles in several important biological events such as gene expression mitotic checkpoint and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation.
In ciliates a unicellular organism having two functionally distinct nuclei GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus and these different Nup98s participate in a nucleus-selective transport mechanism.

抗Cdk1/Cdc2 (Xenopus) 抗体 ウサギポリクロ

製品番号:69-003

Storage： Shipped at 4°C and store at -20℃

Reactivity: Xenopus human

Applications
1) Western blotting (1/200-1/500 dilution)
2) Immunoprecipitation (1/100 dilution)

Immunogen: Synthetic peptide corresponding to C-terminal region of Xenopus Cdk1 protein (C-KSSLPDNQIRN) conjugated with KLH

Purity: Purified IgG

Form: 1.0 mg/ml in 1 x PBS and 50% glycerol

Background: Cdk1(cyclin-dependent kinase1) plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme while phosphorylation at Thr-161 activates it. Molecular mass is 34506

抗Leaf-FNR2抗体、ウサギポリクロ

商品コード:81-003

Background: Ferredoxin-NADP reductase, leaf isozyme 2 (L-FNR2) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.

抗Leaf-FNR3抗体、ウサギポリクロ

商品コード:81-005

Background: Ferredoxin-NADP reductase, leaf isozyme 1 (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.

抗-Nup98抗体、マウスモノクローナル (13c2+21A10)

製品番号:70-347

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition Nup98 plays roles in several important biological events such as gene expression mitotic checkpoint and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation.
In ciliates a unicellular organism having two functionally distinct nuclei GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus and these different Nup98s participate in a nucleus-selective transport mechanism.

抗Nup98 抗体、ラットモノクローン(2H10)

製品番号: 70-310

Nucleoporin 98 (Nup98) は核膜に埋め込まれた大きな構造体である核膜孔(NPC)の構成成分の一つで
あり、核膜孔の両側に存在する。このタンパク質はGly-Leu-Phe-Gly の繰り返し配列を持っていて、タン
パク質の核移行及びRNA の核外への移行に必須である(1)。Nup98 遺伝子は白血病患者の染色体上で転移
していて種々の遺伝子と融合している(2)。このハイブリドーマは大阪市立大学の立花太郎博士の研究室で
作成された(3)。
用途:
1)ウエスターンブロッテイング 2)免疫染色 3)ELISA 4) ドットブロッテイング
性質: 本抗体を細胞質に注入すると核膜孔に蓄積し、内在性のRan タンパク質の核局在を阻害する。
抗原: 組換え体Nup98 タンパク質 (アミノ酸 1-466)